Pseudomonas aeruginosa is an adaptable microorganism that causes an extensive spectrum of infections and intrinsically resistant to many antibiotics. Antimicrobial resistance has increased among these bacteria due to many resistance mechanisms. Antibiotic resistance has become global problem. This study was done to analyse the antibiogram of Pseudomonas aeruginosa at a tertiary care centre, Vadodara. 115 isolates were recovered from various specimens for a period of 6 months from January to June 2023 and antibiotic susceptibility testing was done in Vitek 2 automated machine. Pseudomonas aeruginosa was found to be high (45.9%) in pus/wound than other samples. Out of 115 Pseudomonas aeruginosa isolates, 60 isolates are most sensitive to Piperacillin/Tazobactem (52.17%) followed in decreasing order by Amikacin (51.30%), Cefoperazone/Sulbactam (49.57%), Cefepime (47.83%), Meropenem (46.09%), Imipenem (45.22%), Ciprofloxacin (42,61%), ceftazidime (40%), Levofloxacin (37.39%), Gentamicin (35.65%), Aztreonam (13.04%) and Colistin, Minocyclin, Tigecycline, Trimethoprim/Sulphamethoxazole, fosfomycin (0%).