Pharmacy Practice in the Era of Personalized Medicine: Challenges and Opportunities delves into the transformative landscape of personalized medicine and its profound impact on pharmacy practice. The chapter is structured to provide a comprehensive understanding of this dynamic field. It begins by introducing personalized medicine, emphasizing the significance of tailoring medical decisions to individual characteristics and the evolving role of pharmacists. The purpose is to explore the challenges and opportunities pharmacists face in this context. The chapter delves into the concept of personalized medicine, discussing its principles, historical development, and the shift from a one-size-fits-all approach to individualized treatment. Pharmacogenomics takes center stage, explaining how genetics influence drug responses and how pharmacogenomic testing is being integrated into pharmacy practice. Challenges in pharmacy practice are addressed, encompassing ethical and legal considerations, integration of pharmacogenomic data, and ensuring patient privacy and informed consent. Opportunities and advancements highlight how technology and collaboration with healthcare teams are reshaping pharmacy practice. Pharmacists are central in medication therapy management, ensuring optimal patient outcomes. Education and training are pivotal, with a focus on the requirements for pharmacists in personalized medicine and continuous professional development. This section ensures that pharmacists remain well-equipped to navigate the complexities of pharmacogenomics. Case studies and practical applications offer real-world examples of personalized medicine's impact on patient care, illustrating its transformative power and offering valuable lessons. The future trends and prospects section highlights emerging trends, the potential of AI and machine learning, and the expanding scope of personalized medicine services. Pharmacists are positioned to excel in this evolving field. The chapter concludes by recapitulating key challenges and opportunities, emphasizing the importance of adapting to personalized medicine, and calling pharmacists to embrace and excel in this transformative field. In a rapidly evolving healthcare landscape, pharmacists have the opportunity to lead the way in providing patient-centered, genetics-informed care, ultimately improving healthcare outcomes and patient satisfaction.