Introduction: Surgical Site Infections are defined as infections occurring within 30 days after surgery or within 90 days if an implant is left in place after the procedure and affecting either the incision or deep tissue at the operation site. The rate of SSI in India is 3% to 12%. It is 3rd most common hospital-acquired infection is surgical site infection (SSI). Methodology: A retrospective study of 8 months from January 2022 to August 2022 was done where pus samples were sent to the laboratory from Surgery Department which were clinically diagnosed SSI. Sample were processed in Bacteriology Laboratory and bacteriologically confirmed cases of SSI were included in the study. The culture and AST of the samples were done, and the results were interpreted accordingly. Observation: Out of a total 1063 surgeries in 8 months, 79 were bacteriologically & clinically diagnosed with SSI. So, the prevalence rate was 7.43%. Out of 79 surgeries, 44 were Emergency Surgeries and 35 were Elective surgery. The rate of SSI was more in Emergency Surgeries than Elective surgeries. Discussion: The study helps in creating an antibiotic policy for the hospital. It also helps in reducing the rate of SSI, and infection control policy. Conclusion: The study is used in identifying the organisms and their antibiotic sensitivity pattern in patients with surgical site infections and thus benefited in the treatment. It will help to identify the predisposing factors and thus reduce the infection rate in the post-operative period and thus will also help in reducing the rate of infections and reduce the use of antibiotics.