Aim: The aim of the present was to identify the preferential feeding of Ae. aegypti on human ABO blood groups and their impacts on fecundity. Methods: Separate membrane feeders exposed laboratory-reared female Ae. aegypti mosquitoes to all four blood types. After feeding, mosquito blood DNA was collected and identified using ABO genotyping PCR. Fecundity was measured by egg batch mean egg count. Results: Amplification of the 334bp fragment in the human DNA detection PCR revealed 100 female blood-fed mosquitoes among 205 permitted to feed. The ABO genotyping PCR found blood classes A (18), B (19), AB (25), and O (30). Eight samples also had numerous blood meals. The mean number of eggs deposited per female was evaluated using one-way ANOVA with 0.05 significance. No blood group had substantial fecundity differences. Conclusion: Understanding Ae. aegypti's affinity for blood type O led to the development of a novel adult mosquito attractant trap that can combat pesticide resistance. Blood type O persons have an increased risk of contracting vector-borne illnesses transmitted by Ae. aegypti due to more vector bites.