Molecular markers plays an important role in applied research (e.g. marker assisted selection, paternity testing and food traceability) as well as basic applicability (e.g. phylogenetic analysis and search for useful genes). Diversity among organisms is a result of variations in their genetic profiling and effects of environment i.e. temperature, cold etc. Information grain from the genetic material is essential in optimizing both conservation and utilization of those particular organism/plants. To study the genetic variation such as similarity and dissimilarity among the organism/plants modern techniques (DNA marker, genome editing tools) play a vital role.