Earthworm (also known as earth dragon) has been used for many decades as fever reducer. Crude extract from different species of earthworms after performing fibrin assay revealed its protease property. Earthworm extracted protease performs variety of functions including as an antithrombotic agent and minimizes the platelets aggregation and shows high specific nature for substrates. Enzymes have been extracted from various species of earthworm and these enzymes are collectively called lumbrokinase. Lumbrokinase (LK) activates the plasminogen and dissolves the blood clot that entangles in blood vessels. The experimental and computational studies of lumbrokinase enzymes showed serine protease nature and the amino acid sequence of lumbrokinase is similar with the serine proteases. The domains of lumbrokinase revealed its serine protease nature that belongs to PA clan and trypsin family. To target thrombolysis different scientists introduced different delivery systems for the accurate aiming of lumbrokinase towards the target, LKs loaded small nano micelles have been targeted towards thrombus. Thus, through different analyses it was recorded that extracts of earthworm exhibit different intermediates. The protease provides good hemorheological efficacy and is significant for the treatment of thrombosis.