Surface immobilization of copper hexacyanoferrate (CuHCF) film on gold nanoparticle (GNP) L-Cysteine (L-Cys) capped graphite paraffin wax composite electrode (GWCE) was achieved by a new approach. Thiol group (-SH) of L-Cys was covalently linked to GNP adsorbed on graphite and the terminal amino group (NH2) of L-Cys was utilized to anchor the CuHCF film. The modified electrode fabricated using paraffin wax exhibited an excellent binding ability and robustness. Comparatively a stable and reproducible redox peak at a lesser formal potential of 0.326 mV Vs SCE at a scan rate of 20 mV s-1 in 0.1 M KNO3 was observed for the modified electrode. Preliminary investigation has shown that the surface immobilized CuHCF film on GNP-L-Cys-GWCE exhibited a high catalytic activity for the oxidation of uric acid.