The present new study was carried out to determine the frequency and wavelengths in C. diurnum (C. diurnum) leaves by using molecular spectroscopic instruments (FT-IR spectroscopy and UV-Vis Spectrophotometry). A series of extracts were prepared by using solvents (polar and non-polar solvents) and C. diurnum dried leaves powder. The polar (ethanol, double-distilled water, and ethyl acetate) and the non-polar solvent extracts (hexane, and carbon tetrachloride) put into the FT-IR spectroscopy, give the frequencies at 3330.61 cm-1, 1716.17 cm-1, and 3340.87 cm-1 for polar and frequencies at 1702.86 cm-1 and 1716.17 cm-1 for non-polar extracts. The polar and the non-polar solvent extracts put into the UV-Vis Spectrophotometry give the wavelengths at 538 nm, 608 nm, 647 nm, 677 nm showed for ethanol, 542 nm, 612 nm, and 672 nm showed for double-distilled water, 555 nm, 648 nm, and 680 nm for ethyl acetate for polar solvent extracts. Further, the wavelengths showed at 613 nm, 647 nm, and 682 nm for hexane and 564 nm, 597 nm, and 646 nm for carbon tetrachloride for non-polar solvent extracts. The polar solvent extracts showed -OH stretching frequency for ethanol extract, -OH stretching frequency for double-distilled water extract, and -C=O stretching frequencies (acid) for ethyl acetate extracts, and also the non-polar solvent extracts give the -C=O stretching frequencies (acid) for hexane extract and -C=O stretching frequency (ester) for carbon tetrachloride extract.