About 800 million people suffer from hunger, but even more suffer from micronutrient malnutrition, also called “hidden hunger”, particularly in the developing countries. Iodine, vitamin A, iron, and zinc malnutrition are major concerns. The malnutrition of minerals (Fe, Zn) and vitamin A are major food related primary health problem among populations of the developing world including India where there is a heavy dependence on cereal-based diets and limited access to fruits and vegetables. One such approach to combat the issue of micronutrient malnutrition is through bio fortification, a process of breeding nutrients into food crops which provides a comparatively cost-effective, sustainable, and long-term means of delivering more micronutrients to rural populations in developing countries. Currently, agronomic, conventional, and transgenic bio fortification are three common approaches. Agronomic bio fortification can provide temporary micronutrient increases through fertilizers. In conventional plant breeding, parent lines with high vitamin or mineral levels can be crossed over several generations to produce plants that desired nutrients. Transgenic approaches are advantageous when the nutrient does not naturally exist a crop (example, provitamin A in sweet potato and cassava. Recently, there have been several reports on the development of transgenic crops to enhance levels provitamin A content in crops like tomato, potato, cassava, sweet potato, beans and other vegetable crops.