Neem (Azadirachta indica A. Juss.) is an attractive evergreen tree native to the Indian subcontinent. It has attracted worldwide prominence owing to its wide range of medicinal properties. The research about neem has been focused on its versatile biological activities, antifungal potential being one of them. Neem has been used for a long time in agriculture and alternative medicine. It is considered as a safe medicinal plant having numerous biological properties without any adverse effect. Every part of the plant viz., the leaves, bark, fruit, seeds, kernels, oil, and roots have been reported to have multiple uses. This review summarizes the antifungal role of A. indica in the prevention and management of various fungal diseases of plants, humans and animals.