Medicinal plants are important sources for plant secondary metabolites, which are important for human healthcare. Painkiller (e.g., morphine), cardiotonic (e.g., digoxin), antihypertensive (e.g., reserpine), antitussive (e.g., codeine), antineoplastic (e.g., vinblastine and paclitaxel), or antimalarial (e.g., artemisinin) compounds, are only a few of the several irreplaceable medicinal products derived from plants (Nessler 1994). Ashwagandha, Giloy, Mulethi, and Cinchona are important medicinal plants that are helpful for boosting our immunity to fight with covid-19 and many other diseases (Shree et al. 2020). Improvement of the yield and quality of these natural plant products is still a challenge. The high content of desirable compounds, absence of harmful substances, high dry matter content, and high antimicrobial properties are the major breeding objectives for medicinal plant breeding (De, 2017). Breeding and Research work on medicinal plants have been largely neglected, and thus there is limited knowledge regarding their genetic background, heterozygosity, growth cycle, and self-incompatibility. To achieve this scale of production, breeding studies are necessary for further development of medicinal plant cultivation. Although advances have been made in the breeding of some medicinal plants, several challenges remain, owing to the particularity and complexity in determining the breeding target. Additionally, in medicinal plants, there are limitations associated with research on traditional and modern breeding methods.