Background: The vast biodiversity of North East India is well-known, and the locals there eat differently from those in other parts of the nation. Numerous people in this area considered insects delicacies and used them in their diets. Manipur is a relatively undiscovered region with limited research on the variety of its wildlife, particularly about insects. Although entomophagy and entomotherapy have been practiced for a long time, a thorough description of the nutraceutical potential of edible insects is still missing. To our knowledge, no studies have been carried out on the anti-microbial potential of Hydrous indicus and Gryllotalpa orientalis, two edible insects commonly consumed by the living population of Manipur. Method: Herein, we aim to investigate the anti-microbial potential of Hydrous indicus and Gryllotalpa orientalis for the first time. The Anti-microbial assays include the anti-microbial Inhibition Test of Bacillus subtilis and Escherichia coli, the Anti-fungal Zone Inhibition Test of Candida albicans, and the minimum inhibitory concentration activity of Bacillus subtilis, Escherichia coli and Candida albicans were also studied were carried out. Results: The results reveal that the antimicrobial assays specially antimicrobial zone inhibition test against B.subtilis, E. coli and C. albican, both in H.indicus and G.orientalis these bacteria are at different concentrations. However, the study also reveals that very low Anti-microbial property against B. subtilis was observed in H.indicus and G.orientalis (IC50 = above 1000µg/ml) while Low Anti-microbial property against E. coli was observed in H.indicus and G.orientalis (IC50 = above 1000 µg/ml). However, based on the study, it was observed that G.orientalis exhibited significant anti-microbial activity against C. albicans (IC50 = 0.5107 µg/ml, and MIC=0.05 µg/ml). Conversely, samples H.indicus (IC50 = 427 µg/ml, and MIC= 0.07 µg/ml) showed lower anti-microbial activity against C. albicans than G.orientalis. Conclusion: The results of the study suggest that H. indicus and G. orientalis were found to be low antimicrobial potential and thus can be sustainable and functional foods in the future. As a result, these insects have a significant impact on environmental management, health, and food security.