A total of 56 fecal samples were analyzed for the study of parasite prevalence during October 2018 to November 2019 from Rhesus Macaques Macaca mulatta residing certain areas of Kamrup Metropolitan district of Assam. These samples were analyzed by fecal concentration methods viz. simple test tube flotation technique and sedimentation technique. The result shows that 49 samples were found infected by gastro-intestinal parasite. The study has found 11 gastro- intestinal parasites, of that 7 species were identified that belongs to 7 genera from the 5 family. The percentage occurrences of Helminth (58.16%) were found higher the Protozoa (46.94%). All the samples were found to be infested with one or more species of gastrointestinal parasites. The percentage of occurrence of Ancylostoma sp. (50%) was found highest followed by Enterobius vermicularis (42.86%), Coccidiosis Egg (41.07%), Strongyloides stercoralis (39.29%), Ascaries sp. (35.71%), Ancylostoma duodenal (25.00%), Capillaria philippinensi (23.21%), unidentified spp. 1 (23.21%), unidentified spp. 2 (19.64%), unidentified spp. 3 (17.86%) Ancylostoma egg (14.29%) and unidentified spp.4 (14.29%).