Introduction: Tuberculosis is a widespread, infectious disease caused by various strains of Mycobacteria usually Mycobacterium tuberculosis. Tuberculosis typically attacks the lungs and can also affect other parts of the body. An active TB infected patient’s cough, sneeze, or otherwise transmit through respiratory fluids are the common cause for the TB through the air. Material & Method: This study, which was conducted in tertiary care hospital, Jamnagar. There were 2 sputum samples, one spot supervised and one early morning collected and transported from various centers to TB culture-DST laboratory, Total 97 sputum samples of HIV positive patients were received during the study period and proceeded by GeneXpert MTB/RIF Assay. Result: Out total 97 HIV positive patient’s sputum samples, in 27 (27.8%) samples Mycobacterium tuberculosis bacilli (MTB) were detected. Conclusion: The study revealed the GeneXpert MTB/RIF Assay test offers a potential solution for improving early MTB diagnosis in immunocompromised individual like HIV.