Introduction: The genus “Neoscytalidium” is a member of Botryosphaeriaceae family and containing four species including N. dimidiatum, N. oculus, N. orchidacearum, and N. novaehollandiae. The Fungus Neoscytalidium novahollandiae is an endophytic pathogen and non-dermatophytic fungi. Though, this was isolated from a baobab by Pavlic et al back in 2008, the first human case causing finger nail onychomycosis by N. novaehollandiae in an Iranian female in 2019 was published by Shokoohi et al. Case: A 43-year-old male came to the Dermatology OPD at DGH with the complaints of right-hand finger nail changes for 3 years and a H/O trauma 4 years back while farming. No H/O diabetes or immunocompromised. The nail specimen was sent to the microbiology lab for culture and sensitivity for the better treatment to the patient. Material & Method Direct Microscopy: KOH wet mount preparation of sample showed brownish aseptate conidia in chains. Culture on SDA: Colony was first seen on second day itself on a plate at room temperature. Colony was fast growing within 3 days, under dark condition at room temperature. Colony were initially white, turning greenish and brown with age, exhibiting suppressed, fluffy and powdery mycelium, smooth edges. No colony was seen in a plate which was kept in incubator. Conclusion: As per morphological features seen on KOH mount and gross appearance of the growth, the isolated fungi was suspected to be Neoscytalidium novahollandiae. However due to financial limitations we could not do molecular diagnosis. On our suspicion the patient was received oral terbinafine and ciprofloxacin and responded to the treatment within 3 months.