Background & Objective: The present study was conducted to assess prevalence of Candida in patients of urinary tract infections. Materials and Methods: This retrospective study was carried out over period of 5 months from June 2016 to October 2016 at B.J Medical College, Civil Hospital Ahmedabad, Gujarat. Patients who presented with signs and symptoms of urinary tract infections were included. Urine samples were received from the patients clinically suspected of urinary tract infection. Isolation and identification of pathogen was done by standard microbiological method. Antifungal susceptibility testing for amphotericin-B, itraconazole, clotrimazole, fluconazole was carried out using out standard methods. Result: Out of total 4785 urine samples tested, 168 (3.51%) showed growth of Candida species. Out of these 168 positive patient for Candida, 68(40.4%) was positive for Candida albicans, 56 (33.3%) was positive for Candida tropicalis, 10 (5.95%) was positive for Candida parapsilosis, 27 (16%) was positive for Candida krusei, 7 (4.16%) was positive for Candida dublinensis. The Candida isolates were more susceptible to Amphotericin-B (81.47%) and Itraconazole (60.30%) compared to Clotrimazole (59.60%) and Fluconazole (51.67%). Conclusion: Based on present study there is need to consider candiduria as an important entity in today’s scenario. The presence of candiduria represents therapeutic challenge and should be verified by second clean catch urine culture. It is of utmost importance not only to identify candida species but also to conduct its antifungal profile.