Authentication of herbal medicine through sequencing involves using DNA sequencing techniques to identify and verify the presence of specific plant species or genetic markers in herbal products. This process ensures the quality, safety, and efficacy of herbal medicines by confirming their ingredients. Here are the steps and considerations involved in authenticating herbal medicine through sequencing. By implementing DNA sequencing for herbal medicine authentication, manufacturers, regulators, and consumers can have greater confidence in the quality, safety, and efficacy of herbal products, and it helps prevent fraud and mislabelling within the herbal medicine industry.