More than 20,000 insect species are herbivores, i.e. potential agricultural pests destroying up to 30% of our potential annual harvest. They depend on plants for food and have adapted their metabolism accordingly in the course of evolution to render plant defenses. The operating instructions of detoxification processes are coded in different genes. Knowing genes that allow particular insects as pests and switching them off with the consequence of plant toxins or insect derived molecules is currently a research subject i.e. Yellow bio-technology. 'Yellow biotechnology' refers to biotechnology with insects-gene silencing using RNA interference (RNAi), which is an emerging technology which can be a biologically safe alternative to control insect pests. The technology relies on controlling gene expression and regulation of protein function through small interference RNA (siRNA) tools. Though the tobacco plant, Nicotiana attenuata, produce nicotine as a defensive substance against herbivores, it does not have any toxic effects on their worst enemy, larvae of the tobacco hornworm, Manduca sexta as the insect is resistant against this alkaloid. The genes that encode nicotine-catabolizing enzymes may be responsible for its resistance. These so called CYP genes are involved in the formation of cytochrome P450 enzymes. The DNA sequences of CYP genes were identified in Manduca sexta and switching off these genes using RNAi, gave better results. When the larva was fed with supplemented dsRNA, insect gene CYP6B46, responsible for detoxifying nicotine was silenced. This yellow pest management is involved in disease management in honey bee. To protect honey bee from the infection by the Israeli Acute Paralysis Virus, the Remebee (dsRNA product) used to silence insect gene responsible for the disease, which showed decreased mortality and increased honey production. Synthetic insecticides and transgenic plants producing bt toxins have been proven to be successful in controlling insect pests in agricultural crops but resistance developments and environmental concerns are serious issues. The advantages and promises from RNAi technology sound amazing. Progress made so far in RNAi technology provides an ample evidence for RNAi to become a successful alternate for insect pest management. Despite various doubts associated with this technology, the days are not too far when one would see RNAi would stand along Bt technology in insect pest management programs. This review narrates methods of dsRNA uptakes, promising examples of RNAi technologies against insect pests and challenges for RNAi as successful insect control strategy.