Bio-fortification or biological fortification is a process that is directed toward increasing the level of specific micronutrients and vitamins in edible tissues of crops through agronomic practices, plant breeding, and transgenic approaches. According to estimates, 2.7 billion people are zinc (Zn) deficiency, 4 billion people are iron (I) deficient, and hundreds of millions are one or more essential vitamins (WHO 2002). The three nutrients that are most commonly deficient in diets worldwide are iron, zinc, and vitamin A recognized by World Health Organization (WHO). The main focus of bio-fortification research is iron, zinc, and vitamin A deficiencies. Despite having “enough eat” many people may not consume enough food to provide adequate nutrition. The result is the issue of “hidden hunger”. Micronutrient deficiencies, such as those in zinc and iron, are referred to as “hidden hunger” or also known as “micronutrient malnutrition”. The objectives of biofortification are the improvement of protein quality and content, oil quality and content, vitamin content, micronutrients, and mineral content. Agronomic bio-fortification is the application of micronutrients containing mineral fertilizer to the soil or plant leaves for increasing micronutrient contents of the edible part of a food crop. In conventional plant breeding techniques, parent lines with high nutrient content are crossed with recipient lines with desirable agronomic features over several generations to generate plants with the desired agronomic and nutritional characteristics. New breeding techniques i.e., Transgenic breeding, RNA interference (RNAi), and genomic editing are playing important role in the biofortification of crops by the creation of new genetic variation that does not exist in the gene pool.