Environmental concerns related to textile industry have attained attention from all around the world as it is generating large amounts of environmental pollution and effluents having various toxic agents and chemicals. With the increasing awareness of environmental pollution and the extensive consideration of mankind health and chemical processes used in textile industries are being replaced by bio-processing. Bio-processing of textiles has become a preferred choice to overcome the effects of chemical treatment because of being less harmful to environment as well as textiles. Usages of enzymes in textile industry can be the best possible example of bio-processing. Use of enzymatic treatments in textile industries is very promising approach as they are eco-friendly, produce high-quality products, and lead to the reduction of energy, water, and time. This review highlights about the various bio-processing techniques used in the textile industry such as bio-retting, bio-desizing, bio-scouring, bio-bleaching, bio-polishing, bio-stoning, degumming of silk, shrink-proofing for wool and many more.