Because of their distinctive physical and chemical properties, silver nanoparticles (AgNPs) are rapidly being used in a variety of industries, including medicine, food, health care, consumer goods, and industry. Among these are optical, electrical and thermal properties, as well as strong electrical conductivity and biological qualities. The current work validates the potential of Vigna unguiculata plant extract to biosynthesize silver nanoparticles (Ag NPs). The findings of surface plasmon resonance at 450 nm proved the creation of Ag NPs. Furthermore, SEM revealed that the nanoparticles exhibited a spherical form. Antimicrobial assays confirmed the bactericidal efficacy of biosynthesized Ag NPs against Gram-positive and Gram-negative bacteria, as well as the antioxidant activity. According to the findings, the Vigna unguiculata extract-mediated AgNPs have the potential to attract metallic nanoparticles for the up-scaling of metallic nanoparticles in order to investigate various catalytic and biological applications.