Recent Trends in Molecular Biology and Biotechnology (Volume - 5), edited by Dr. Yogendra Singh, offers a detailed exploration of molecular innovations with practical implications in agriculture and biotechnology. This volume contains seven comprehensive chapters on genomics in seed vigor and longevity, antisense RNA and RNAi in gene silencing, anhydrobiosis in nematodes, pharmacological potential of plant secondary metabolites, marker-assisted selection, CRISPR-Cas9 genome editing, and plant tissue culture. Featuring case studies and experimental evidence, it highlights key techniques such as QTL mapping, RNA interference, genetic transformation, and molecular breeding strategies. Designed for researchers, students, and agri-biotech professionals, the volume serves as a vital academic tool for understanding genetic enhancement, disease resistance, and sustainable crop development. With a balanced mix of foundational knowledge and emerging trends, it bridges theoretical frameworks with real-world applications in biotechnology.