Recent Trends in Molecular Biology and Biotechnology (Volume - 7), edited by Dr. Yogendra Singh, features five high-impact chapters that explore state-of-the-art advancements in gene editing, diagnostic technologies, and sustainable crop improvement. This volume includes detailed research on next-generation PCR and isothermal amplification techniques, the kinetic mechanism of CRISPR/Cas9 for precision genome editing, detection methods for biotic stress in plants, genomics-assisted breeding for sustainability, and the prospective role of CRISPR/Cas9 in agriculture. The book highlights applications such as Loop-Mediated Isothermal Amplification (LAMP), Rolling Circle Amplification (RCA), and marker-assisted selection while also addressing cancer treatment and immunotherapy using CRISPR. By integrating molecular tools with agricultural and clinical applications, this volume is an essential reference for students, molecular biologists, plant scientists, and biotechnologists focused on innovation in diagnostics and crop genomics.