Introduction: Chikungunya infection is re-emergence public health problem globally. Chikungunya is an arthropod-borne disease transmitted by mosquitoes Aedes aegypti. This study is conducted to know the seroprevalence, seasonal trends of chikungunya infection. Chikungunya is RNA virus that belongs to the alphavirus genus of family Togaviridae. This study was conducted at Microbiology Laboratory of Tertiary care Hospital from January to December 2020-2021. Serum sample from Chikungunya Suspected patients were tested for immunoglobin M (Ig M) antibodies by ELISA method. Aim and Objectives: The present study was to observe the seroprevalence of chikungunya among suspected patients admitted with history of fever, headache, joint pain in Sir T Hospital Bhavnagar. Material and Method: The present study included serum samples of suspected patient of Tertiary care Hospital Bhavnagar for the period of January 2020 to December 2021. Serum was separated and subjected to ELISA (Enzyme Linked Immunosorbent Assay) method for the detection of Ig M antibodies against chikungunya virus infection. Result: Seroprevalence is 17.77% in 2020 and 39.59% in 2021. The significant rise in detection of cases is seen in year 2021 in comparison of previous year. Seropositivity is 13.55% in 0-20 year, 47.45% in 21-40 year, 28.81% in 41-60 year, 10.16% in >60 year during January 2021 to December 2021. Conclusion: This Study shows seasonal trends during winter months as the cases rises during September to December. Seropositivity is higher among 21-40 year of age (47.45%). This suggests to take necessary preventive steps to control vector breeding places before these months and other preventive steps and prepare the infection management algorithm.