DNA sequencing is the determination of the precise sequence of nucleotides (Cytocine, Thymine, Adenine and Guanine). Chemical degradation and chain termination methods of sequencing were conventional sequencing methodologies to determine the sequences in genomes. In 1987, Applied Biosystems introduced the first automated sequencing technology based on capillary electrophoresis. Next-generation sequencing methods further enhanced sequencing data with reduced cost. The technique of sequencing is experiencing a revolution, which is concurrently generating a revolution in genomics and genetics. Numerous species, including economically valuable crops, have had their whole genomes sequenced using next-generation sequencing/2 generation sequencing (NGS/2GS) techniques. Genomic techniques and resources are leading to a new plant breeding revolution. These methodologies allow for the investigation of the genotype and its link to the phenotype, particularly for complex characteristics. A thoroughly sequenced genome provides essential tools to breeders since it enables the identification of genes.