The generation of new plantlets with desired characters is a laborious and expensive technique. Genome-editing technology provides an advanced biotechnological tool that enables a precise and efficient targeted modification of an organism’s genome. They have been utilized in a wide variety of plant species for the characterization gene as well as to improve desirable traits in agricultural crops, in particular. Among the gene editing tools, Clustered Regularly Interspaced Short Palindromic Repeats and associated protein 9 (CRISPR-Cas9) provides a technological breakthrough in targeted mutagenesis for the improvement of crops The application of CRISPR/Cas9 and related technologies in plant genome engineering will not only facilitate crop breeding but also accelerate the progress for generating transgene-free genome edited plants. The present paper highlighted the various aspects of CRISPR/Cas9 technology in plants and its applications in crop improvement.