Agriculture Biotechnology is one of the basic tools to increase crop production and make agriculture sustainable. As opposed to the conventional plant breeding strategies, biotechnological tools like tissue culture, genetic engineering, transgenesis, recombinant DNA technology are more effective in designing new crops. The focus is to make maximum utilization of available resources without exhausting them. Earlier crop yields were increased by incessant use of fertilizers and pesticides, which not only degraded the environment but also released many harmful chemicals than can enter the food chain and cause deleterious effects. So it is very important that the anthropogenic activity does not destroy, rather has the potential for long term maintenance of the natural world and its resources. Environmental sustainability can be attained if the current processes of interaction with the environment are done in a more thoughtful way without recklessly depleting them. An unsustainable situation arises when the natural capital is being used up faster than it can be restored. Such degradation on global scale can bring the end of civilization. This can be seen as nature’s fury in the form of global warming, melting of glaciers, and rise of water level, torrential rains and cyclones. This leads to severe loss of food crops, ghastly affects crop yield and production. So we have to adhere to polyculture, organic farming as well as grow genetically modified crops which are stress tolerant and resistant to pests. Genetically Modified crops have their share of shortcomings, so risk assessment has to be done before importing any crop for cultivation. Sustainable agriculture is the need of the hour which can only be achieved by using biotechnological tools to design GM crops and practicing organic farming.