Nowadays, biotechnology has been applied in every sphere of life and recently many advances in food industry represent great role of food biotechnology. In the interest of a sustainable food supply for the accelerated global population, food biotechnology has emerged as a robust tool for upgrading and increasing food production and bioprocessing. It plays a prospective role in various food processing sectors, such as fruit and vegetables, beverages, cereal, dairy, oils and fats, poultry, and confectionery. Food-based technologies established itself as a vast, interdisciplinary research area which is at crossroads of scientific and technological advancements. Biotechnology as a science deal with the applicability of biological systems, living organisms or derivatives thereof, to make or modify products or processes for specific use. The applications and practices of biotechnology in food industry is immense and has the following applications such as – its consumption in fermentation of elements and also to enhance assets of the factual goods such as the taste, perfume, shelf-life, surface, quality and nutritional value of that exact food product. Biotechnology has a vital role in the creation of enzymes and the use of firm enzymes leads to the vital modifications in food. The recent times has seen the application in field of flavor change, aroma, food essences and an array of other high valued-enhanced good as food additives. It has brought remarkable changes in field of food processing in production of products with longer storage life and nutritive value. The usage of biotechnology mainly aims at producing genetically modified foods and genetically modified starter culture. Flavour enhancement and production of vaccines are another important aspect covered under application of biotechnology in food processing.