The core of the infection strategy of pathogenic Agrobacterium is an inheritable metamorphosis of the host cell, via stable integration into the host genome of a DNA particle called T-DNA. This inheritable metamorphosis results in the oncogenic reprogramming of the host to the benefit of the pathogen. Therefore, the artificial host range of Agrobacterium is continuously expanding and includes factory and non-plant organisms. The adding vacuity of genomic tools encouraged genome-wide checks of T- DNA tagged libraries, and the pattern of T-DNA integration in eukaryotic genomes was studied. Thus, data have been collected in multitudinous laboratories to attain a better understanding of T-DNA integration mechanisms and implicit impulses. It focuses on the intranuclear mechanisms necessary for proper targeting and stable expression of Agrobacterium oncogenic T-DNA in the host cell. More specifically, the part of genome features and the apparent involvement of the host's transcriptional ministry concerning the T-DNA integration and goods on gene expression are bandied. Also, the mechanisms underpinning T-DNA integration into specific genome chambers are reviewed, and a theoretical model for T-DNA intranuclear targeting is presented.